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Schematic representation of the study design, <t>microarray</t> protocol, and data analysis. ( a ) Sample acquisition and heat inactivation of virus, ( b ) SARS-CoV-2 whole proteome microarray design, ( c ) microarray staining and image acquisition, ( d ) data analysis pipeline.
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Schematic representation of the study design, microarray protocol, and data analysis. ( a ) Sample acquisition and heat inactivation of virus, ( b ) SARS-CoV-2 whole proteome microarray design, ( c ) microarray staining and image acquisition, ( d ) data analysis pipeline.

Journal: Viruses

Article Title: Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray

doi: 10.3390/v15010248

Figure Lengend Snippet: Schematic representation of the study design, microarray protocol, and data analysis. ( a ) Sample acquisition and heat inactivation of virus, ( b ) SARS-CoV-2 whole proteome microarray design, ( c ) microarray staining and image acquisition, ( d ) data analysis pipeline.

Article Snippet: PEPperCHIP ® Peptide Microarray slides were brought to room temperature, assembled onto the PEPperCHIP ® incubation tray (PEPperPRINT GmbH, Germany), and equilibrated using the staining buffer for 15 min.

Techniques: Microarray, Virus, Staining

Heatmaps for IgA and IgG response showing major immunogenic regions identified in the SARS-CoV-2 whole proteome microarray. The printed proteome constitutes ORF1a/b polyprotein encoding 16 non-structural proteins (1–10 and 12–16), structural proteins (S, N, E, and M), and the accessory proteins (ORF3a, 6, 7a, 8, and 10).

Journal: Viruses

Article Title: Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray

doi: 10.3390/v15010248

Figure Lengend Snippet: Heatmaps for IgA and IgG response showing major immunogenic regions identified in the SARS-CoV-2 whole proteome microarray. The printed proteome constitutes ORF1a/b polyprotein encoding 16 non-structural proteins (1–10 and 12–16), structural proteins (S, N, E, and M), and the accessory proteins (ORF3a, 6, 7a, 8, and 10).

Article Snippet: PEPperCHIP ® Peptide Microarray slides were brought to room temperature, assembled onto the PEPperCHIP ® incubation tray (PEPperPRINT GmbH, Germany), and equilibrated using the staining buffer for 15 min.

Techniques: Microarray